Optimization of lipid compound synthesis in Theobroma cacao L. in vitro cultures through stress inducers: towards a sustainable approach
Synopsis
Theobroma cacao L., particularly the National “fino de aroma” variety, represents a strategic genetic resource for Ecuador; however, its cultivation is constrained by diseases and climate variability. This study aimed to characterize the fatty acid profile in biotechnological systems exposed to stress inducers (methyl jasmonate, salicylic acid, glycerol, and elevated temperature). Biomass proliferation was optimized in MS, B5, and WPM media, with WPM supplemented with NAA and KIN proving the most effective. Lipid extraction and quantification were conducted through acid hydrolysis, hexane extraction, and FTIR spectroscopy, focusing on oleic, palmitic, and stearic acids. Results indicated that salicylic acid and methyl jasmonate markedly enhanced fatty acid accumulation, whereas glycerol and high temperature had comparatively limited effects. These findings highlight the potential of stress inducers as effective tools to modulate lipid biosynthesis in cacao, offering promising applications for sustainable plant biotechnology.
Published
April 3, 2026
Categories
Copyright (c) 2026 Zulay Marina Niño Ruiz, Laura Inés Salazar Cotugno (Coordinador de obra); Patricia Yissel Dávalos Cerrón, Azucena Chanatasig, Felipe Cerrera, Andrea Carolina Palacios Rosado, Mateo Patricio Sánchez Sanaguano, Liliana Paulina Lalaleo Córdova, William Calero Cáceres, Santiago Casado, Joshua Pazmiño, Jaime Martí-Herrero, Rocio Cecibel Jiménez Paute, Kevin Campaña, Elena Coyago-Cruz, Andrea Hidalgo-Dávila, Lizbeth Alpusig-Guanoluisa, Belén Apolo, Felipe Rivera-Rueda, Yerovi Sánchez-Arias, Karen Marcella García, Rubianith Suárez, Harol Smith Daza, Geovanna Tafurt, Duverney de Jesús Chaverra, Edita Valle, Evelyn Oña, Mishel Calero, Jonathan Liria, Andrés Sebastian Calderón Velasco, Roque Rivas-Párraga, Inti Llumiquinga, Ana Bravo, Julio Rea, Máximo Moreira, Oscar Vivanco, Nicole Jara, Lilibeth Carrión, Stefanía Cevallos, Paulo Herrera, Esteban Patricio Romero Valladares, Ana Paula Rosero-Jácome, Eliza Jara-Negrete, Gabriel Picón Nava, Nelly Paredes, Wilfredo Ramiro Franco, Wilmer Shiguango, Willin Álvarez, Juan Ponce, Jessica Sánchez, Christian Israel Andrade Romero, Alexandra Vargas, David Sanchez, Negover Briñez, Byron García, Dennis Tomalá, Mery Ramirez, Jenny Rodríguez, Paúl Tufiño Mateus, Enrique Laaz, Willan Revelo, Cecilia Puertas, Juan Andrés Guadamud, Karina Olalla-Tacuri , Darwin Omar Yánez Avalos, Diana Callejas, Sara Álvarez, John Mauricio Castillo Torres, Edith Pamela Tapia Pruna, Daniela Endara, Carolina Castro, Silvia Alejandra Llerena Gordillo, Kevin Stwart Tulcanaza Ruiz, Noroska G.S. Mogollón, Melany Ochoa, María Cristina Peñuela, Jimmy Yumbo, Enith Yánez, Carolina Ñacato, Samira Lluno, Henry Conteron, Pedro Cadena, Corina Campos, Oswaldo Guzman, Micaela Campos, Cindy Vera, Dayana Vera, Christian Coral, Bruce Tumbaco, María Soledad Solorzano, Bryan Valencia, José Sebastián Araujo Soria, Sandra Torres, Enzo Loor, Oscar Ayala, Darwin Rosero, Amparo Condor, Luis Cáceres, Miguel Flores, Elena Ulcuango, Nicole Pilco, David Galarza, Mishell Cabrera, Julieth Chancay, Paola Soto, David Portillo, Diana Cisneros, José Aguirre-Déleg, Pablo Maita-Zambrano, Aimee Delgado-Cruz, Myrian Larco, María Soledad Salazar, María Susana Grijalva, Viviana Stefanía Cárdenas Loor, Mónica Dazzini Langdon, Sheila Tobar Vallejo, Vinicio Velásquez Zambrano, Juan Carlos Soria Aguas (Autor de capítulo)
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
How to Cite
Optimization of lipid compound synthesis in Theobroma cacao L. in vitro cultures through stress inducers: towards a sustainable approach. (2026). In III International Congress of Biotechnology and Neotropical Ecosystems (p. e3). Editorial Unión Científica. https://doi.org/10.63804/CIBEN.25.bbfs.e3
