Optimization of plant biofactories of Brassica oleracea var. capitata f. rubra to produce anthocyanins with antioxidant and anti-inflammatory potential through cultivation and genetic transformation strategies
Synopsis
The growing demand for natural bioactive compounds for health and functional nutrition has driven the development of sustainable plant-based production systems. This study aimed to optimize the germination, in vitro culture, and genetic transformation of Brassica oleracea var. capitata f. rubra to increase the accumulation of anthocyanins, phenolic pigments with notable antioxidant and anti-inflammatory properties. Seeds were surface disinfected, cold stratified, and treated with gibberellic acid to enhance germination, and then cultured on Murashige and Skoog medium, both complete and nitrate-free, supplemented with the biotic elicitors methyl jasmonate and salicylic acid. Genetic transformation using Agrobacterium rhizogenes generated hairy roots expressing the transcription factor AtMYB12 and the reporter gene β-glucuronidase (GUS). Nitrate deprivation significantly increased anthocyanin content and antioxidant and anti-inflammatory activities in seedlings, whereas AtMYB12 expression in transformed roots produced an additional increase in pigment concentration and bioactivity. These findings demonstrate that combining nutrient stress with genetic engineering is an effective strategy to enhance bioactive anthocyanin production in purple cabbage, providing a sustainable platform for applications in the food, pharmaceutical, and cosmetic industries and contributing to the conservation of valuable plant genetic resources.
Published
April 3, 2026
Categories
Copyright (c) 2026 Zulay Marina Niño Ruiz, Laura Inés Salazar Cotugno (Coordinador de obra); Patricia Yissel Dávalos Cerrón, Azucena Chanatasig, Felipe Cerrera, Andrea Carolina Palacios Rosado, Mateo Patricio Sánchez Sanaguano, Liliana Paulina Lalaleo Córdova, William Calero Cáceres, Santiago Casado, Joshua Pazmiño, Jaime Martí-Herrero, Rocio Cecibel Jiménez Paute, Kevin Campaña, Elena Coyago-Cruz, Andrea Hidalgo-Dávila, Lizbeth Alpusig-Guanoluisa, Belén Apolo, Felipe Rivera-Rueda, Yerovi Sánchez-Arias, Karen Marcella García, Rubianith Suárez, Harol Smith Daza, Geovanna Tafurt, Duverney de Jesús Chaverra, Edita Valle, Evelyn Oña, Mishel Calero, Jonathan Liria, Andrés Sebastian Calderón Velasco, Roque Rivas-Párraga, Inti Llumiquinga, Ana Bravo, Julio Rea, Máximo Moreira, Oscar Vivanco, Nicole Jara, Lilibeth Carrión, Stefanía Cevallos, Paulo Herrera, Esteban Patricio Romero Valladares, Ana Paula Rosero-Jácome, Eliza Jara-Negrete, Gabriel Picón Nava, Nelly Paredes, Wilfredo Ramiro Franco, Wilmer Shiguango, Willin Álvarez, Juan Ponce, Jessica Sánchez, Christian Israel Andrade Romero, Alexandra Vargas, David Sanchez, Negover Briñez, Byron García, Dennis Tomalá, Mery Ramirez, Jenny Rodríguez, Paúl Tufiño Mateus, Enrique Laaz, Willan Revelo, Cecilia Puertas, Juan Andrés Guadamud, Karina Olalla-Tacuri , Darwin Omar Yánez Avalos, Diana Callejas, Sara Álvarez, John Mauricio Castillo Torres, Edith Pamela Tapia Pruna, Daniela Endara, Carolina Castro, Silvia Alejandra Llerena Gordillo, Kevin Stwart Tulcanaza Ruiz, Noroska G.S. Mogollón, Melany Ochoa, María Cristina Peñuela, Jimmy Yumbo, Enith Yánez, Carolina Ñacato, Samira Lluno, Henry Conteron, Pedro Cadena, Corina Campos, Oswaldo Guzman, Micaela Campos, Cindy Vera, Dayana Vera, Christian Coral, Bruce Tumbaco, María Soledad Solorzano, Bryan Valencia, José Sebastián Araujo Soria, Sandra Torres, Enzo Loor, Oscar Ayala, Darwin Rosero, Amparo Condor, Luis Cáceres, Miguel Flores, Elena Ulcuango, Nicole Pilco, David Galarza, Mishell Cabrera, Julieth Chancay, Paola Soto, David Portillo, Diana Cisneros, José Aguirre-Déleg, Pablo Maita-Zambrano, Aimee Delgado-Cruz, Myrian Larco, María Soledad Salazar, María Susana Grijalva, Viviana Stefanía Cárdenas Loor, Mónica Dazzini Langdon, Sheila Tobar Vallejo, Vinicio Velásquez Zambrano, Juan Carlos Soria Aguas (Autor de capítulo)
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How to Cite
Optimization of plant biofactories of Brassica oleracea var. capitata f. rubra to produce anthocyanins with antioxidant and anti-inflammatory potential through cultivation and genetic transformation strategies. (2026). In III International Congress of Biotechnology and Neotropical Ecosystems (p. e1). Editorial Unión Científica. https://doi.org/10.63804/CIBEN.25.bbfs.e1
