Expression of Penaeidins in Shrimp Penaeus vannamei Treated with Probiotics
Synopsis
Penaeidins are one of the antimicrobial peptides (AMPs) that act as essential components of the shrimp immune system, playing a key role in host defense against bacteria and viruses. This study evaluated the effect of a probiotic consortium composed of Vibrio diabolicus (Ili), Vibrio hepatarius (P62), and Bacillus cereus ss (P64) on the immune response and survival of juvenile Penaeus vannamei challenged with pathogenic Vibrio parahaemolyticus. Juvenile shrimp were cultured for 50 days, applying the bacterial consortium twice daily. Subsequently, shrimp were challenged with Vibrio parahaemolyticus (PirA/PirB) for 48 hours. Penaeidin expression was analyzed through in situ hybridization (ISH) after the challenge, revealing positive infiltrated hemocytes in the connective tissue of various organs, particularly in the buccal appendages, stomach, lymphoid organ, and hepatopancreas. The survival rate of juvenile shrimp was 88.9 %, compared to 22.1 % in the control group, demonstrating the positive influence of probiotic bacteria. In conclusion, the probiotic bacterial consortium exhibited a significant protective effect against vibriosis in Penaeus vannamei culture when challenged with Vibrio parahaemolyticus.
Published
April 3, 2026
Categories
Copyright (c) 2026 Zulay Marina Niño Ruiz, Laura Inés Salazar Cotugno (Coordinador de obra); Patricia Yissel Dávalos Cerrón, Azucena Chanatasig, Felipe Cerrera, Andrea Carolina Palacios Rosado, Mateo Patricio Sánchez Sanaguano, Liliana Paulina Lalaleo Córdova, William Calero Cáceres, Santiago Casado, Joshua Pazmiño, Jaime Martí-Herrero, Rocio Cecibel Jiménez Paute, Kevin Campaña, Elena Coyago-Cruz, Andrea Hidalgo-Dávila, Lizbeth Alpusig-Guanoluisa, Belén Apolo, Felipe Rivera-Rueda, Yerovi Sánchez-Arias, Karen Marcella García, Rubianith Suárez, Harol Smith Daza, Geovanna Tafurt, Duverney de Jesús Chaverra, Edita Valle, Evelyn Oña, Mishel Calero, Jonathan Liria, Andrés Sebastian Calderón Velasco, Roque Rivas-Párraga, Inti Llumiquinga, Ana Bravo, Julio Rea, Máximo Moreira, Oscar Vivanco, Nicole Jara, Lilibeth Carrión, Stefanía Cevallos, Paulo Herrera, Esteban Patricio Romero Valladares, Ana Paula Rosero-Jácome, Eliza Jara-Negrete, Gabriel Picón Nava, Nelly Paredes, Wilfredo Ramiro Franco, Wilmer Shiguango, Willin Álvarez, Juan Ponce, Jessica Sánchez, Christian Israel Andrade Romero, Alexandra Vargas, David Sanchez, Negover Briñez, Byron García, Dennis Tomalá, Mery Ramirez, Jenny Rodríguez, Paúl Tufiño Mateus, Enrique Laaz, Willan Revelo, Cecilia Puertas, Juan Andrés Guadamud, Karina Olalla-Tacuri , Darwin Omar Yánez Avalos, Diana Callejas, Sara Álvarez, John Mauricio Castillo Torres, Edith Pamela Tapia Pruna, Daniela Endara, Carolina Castro, Silvia Alejandra Llerena Gordillo, Kevin Stwart Tulcanaza Ruiz, Noroska G.S. Mogollón, Melany Ochoa, María Cristina Peñuela, Jimmy Yumbo, Enith Yánez, Carolina Ñacato, Samira Lluno, Henry Conteron, Pedro Cadena, Corina Campos, Oswaldo Guzman, Micaela Campos, Cindy Vera, Dayana Vera, Christian Coral, Bruce Tumbaco, María Soledad Solorzano, Bryan Valencia, José Sebastián Araujo Soria, Sandra Torres, Enzo Loor, Oscar Ayala, Darwin Rosero, Amparo Condor, Luis Cáceres, Miguel Flores, Elena Ulcuango, Nicole Pilco, David Galarza, Mishell Cabrera, Julieth Chancay, Paola Soto, David Portillo, Diana Cisneros, José Aguirre-Déleg, Pablo Maita-Zambrano, Aimee Delgado-Cruz, Myrian Larco, María Soledad Salazar, María Susana Grijalva, Viviana Stefanía Cárdenas Loor, Mónica Dazzini Langdon, Sheila Tobar Vallejo, Vinicio Velásquez Zambrano, Juan Carlos Soria Aguas (Autor de capítulo)
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
How to Cite
Expression of Penaeidins in Shrimp Penaeus vannamei Treated with Probiotics. (2026). In III International Congress of Biotechnology and Neotropical Ecosystems. Editorial Unión Científica. https://doi.org/10.63804/CIBEN.25.icta.e3
