Experiences in in vitro propagation of vanilla, a species with potential use in the amazon
Synopsis
Vanilla is an orchid of great economic and cultural value. In Ecuador, Vanilla tahitensis and V. planifolia are cultivated as introduced species, while V. odorata is native to the Amazon. In several areas, production depends on wild populations, which threatens ecosystems and the conservation of germplasm. In vitro propagation offers a sustainable alternative. This study evaluated the in vitro introduction and propagation of V. odorata (from Tena) and V. tahitensis (from Santo Domingo) from seeds. V. odorata did not respond to the disinfection treatments, whereas V. tahitensis showed high germination and development in 50% MS medium supplemented with specific additives and a disinfection protocol using water, alcohol, and chlorine with Tween 20. Seedlings were acclimatized in a substrate composed of moss, perlite, and compost. The in vitro culture of V. tahitensis demonstrates potential for mass production, while V. odorata requires specific disinfection protocols.
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