Experiences in in vitro propagation of vanilla, a species with potential use in the amazon

Authors

Máximo Moreira
Universidad Técnica Particular de Loja, UTPL
https://orcid.org/0000-0002-1833-2674
Oscar Vivanco
Universidad Técnica Particular de Loja, UTPL
https://orcid.org/0000-0001-7799-6297
Lilibeth Carrión
Universidad Técnica Particular de Loja, UTPL
https://orcid.org/0000-0003-4435-7135
Stefanía Cevallos
Universidad Técnica Particular de Loja, UTPL
https://orcid.org/0000-0001-5638-5112
Paulo Paulo Herrera
Universidad Técnica Particular de Loja, UTPL
https://orcid.org/0000-0002-3802-4468

Synopsis

Vanilla is an orchid of great economic and cultural value. In Ecuador, Vanilla tahitensis and V. planifolia are cultivated as introduced species, while V. odorata is native to the Amazon. In several areas, production depends on wild populations, which threatens ecosystems and the conservation of germplasm. In vitro propagation offers a sustainable alternative. This study evaluated the in vitro introduction and propagation of V. odorata (from Tena) and V. tahitensis (from Santo Domingo) from seeds. V. odorata did not respond to the disinfection treatments, whereas V. tahitensis showed high germination and development in 50% MS medium supplemented with specific additives and a disinfection protocol using water, alcohol, and chlorine with Tween 20. Seedlings were acclimatized in a substrate composed of moss, perlite, and compost. The in vitro culture of V. tahitensis demonstrates potential for mass production, while V. odorata requires specific disinfection protocols.

Published

April 3, 2026

How to Cite

Experiences in in vitro propagation of vanilla, a species with potential use in the amazon. (2026). In III International Congress of Biotechnology and Neotropical Ecosystems (p. bbfs.e12). Editorial Unión Científica. https://doi.org/10.63804/CIBEN.25.bbfs.e12